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6 Stage rotation
6
Stage rotation
6.1
Stage with Rotating Mechanism
Loosen the rotation clamp screw on the bottom surface of the stage to allow the stage to swivel.
Rotation clamp screw
6.2
Centering the Stage
(1) Move the 10x objective into the optical path and focus on the specimen. Select a specific point on the specimen as a reference point, then move the reference point to the center of the field of view using the stage knobs.
180° rotation
Reference point
Midpoint of distanced traveled by reference point
(2) Loosen the stage rotation clamp screw and rotate the stage approximately 180°.
(3) If the reference point deviates from the center of the field of view, insert the hexagonal screwdriver into the stage-centering screws* (2 locations) and adjust by moving the stage a distance equal to the distance traveled by the reference point from the center of the field of view.
(4) Using the stage knobs, return the reference point to the center of the field of view.
(5) Move the 40x objective into the optical path and repeat steps 2 through 4.
*
The stage-centering screws (also used as mounting screws) are located at two points on the back of the elevating section.
7
Selection of Magnification
To change magnification, turn the revolving nosepiece and set the desired objective into the optical path.
If the DIH-M is mounted to the microscope, the zoom knob on the right side allows adjustment of magnification between 0.8x and 2x.
Clockwise rotation of the zoom knob increases magnification. The number at the pointer indicates the effective magnification. The knob clicks at each
0.2x increment.
Zoom knob
Total magnification is calculated as follows:
Magnification of eyepiece lens x Magnification of objective x Magnification of zoom knob
18
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Table of contents
- 5 Abbreviations Used in The Manual
- 6 How to use this instruction manual
- 7 Contents
- 11 ON/OFF
- 11 Turning on the microscope
- 11 Supply
- 11 Turning on the fluorescence attachment light source (mercury lamp)
- 12 Adjustment
- 12 Adjustment using the brightness control knob
- 13 Adjustment using the preset switch
- 13 Adjustment with the ND filter IN/OUT lever
- 13 Adjustment with the ND filter for the fluorescence attachment
- 14 Transmitted image in fluorescence observation
- 14 Adjusting the Epi-fluorescence Aperture Diaphragm
- 14 Camera adjustment (adjusting the brightness of the image on the monitor)
- 15 Optical Path Switching
- 15 distribution
- 16 Disabling the clicking of the optical path switching lever
- 17 Motion
- 17 actions
- 17 Knob rotation direction and stage motion direction
- 17 Number of knob turns and distance of stage travel
- 18 Adjusting the rotating torque of the coarse focus knob
- 18 How to Use the Coarse Focus Upper-limit Clamp
- 19 Lateral Stage Motion
- 19 action
- 19 Knob rotation direction and stage motion direction
- 19 Adjusting the knob heights
- 19 Adjusting the knob rotation torque
- 20 rotation
- 20 Stage with Rotating Mechanism
- 20 Centering the Stage
- 20 Magnification
- 21 Adjustment
- 22 Adjusting the Observation Position
- 23 Adjusting the Condenser Position
- 24 Adjusting the Aperture Diaphragm
- 24 12.1 Adjusting the aperture diaphragm opening using the condenser scale
- 25 Selecting a Condenser
- 25 Adjusting the Field Diaphragm
- 26 Operation
- 27 Immersion
- 28 Observation
- 28 17.1 Warning
- 28 17.2 Epi-illumination attachment shutter
- 28 17.3 Light shielding plate
- 29 17.4 Field diaphragm of the Epi-illumination attachment
- 30 Diaphragm
- 31 17.6 Switching Excitation Methods
- 32 17.7 Epifluorescent ND Filters
- 33 Filters
- 33 18.1 Selecting excitation filters (EX filters)
- 34 18.2 Selection of barrier filter (BA filter)
- 35 18.3 Replacing excitation and barrier filters
- 36 Excitation Light Balancer
- 38 Capture
- 38 20.1 Adjusting light intensity
- 38 20.2 Adjusting the condenser
- 38 20.3 Confirming the photomicrographic range
- 38 focus
- 38 20.5 Making adjustments to keep out extraneous light
- 39 photomicrography