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20 Image Capture
20
Image Capture
Images can be captured by mounting a camera head to the ergonomic binocular tube or trinocular eyepiece tube.
For more detailed discussion of this topic, refer to the operating manual provided with the camera head or camera control software.
Proper adjustment of light intensity and focus on the microscope side are important for obtaining clear images. Listed below are key considerations in capturing clear images.
20.1
Adjusting light intensity
Lamp voltage: When the 50i is used in applications for which accurate color reproduction is critical, set the brightness control knob at a midpoint and use ND filters to make brightness adjustments.
Filter: Place a commercially available color compensation filter on the filter holder at the microscope base, as necessary.
20.2
Adjusting the condenser
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Always focus and center the condenser.
Center the annular diaphragm for phase contrast microscopy.
For normal operations, set the diaphragm aperture to 70 to 80% of the N.A. of the objective.
20.3
Confirming the photomicrographic range
The image on the monitor represents the photomicrographic range.
20.4
Confirming focus
Check focus by viewing through the eyepiece and viewing the monitor. If the focal positions for the two images differ, adjust the focal position adjustment screw at the camera port.
20.5
Making adjustments to keep out extraneous light
Field diaphragm: Stop down the diaphragm to a setting just slightly wider than the area shown on the monitor.
Eyepiece: Cover the eyepiece with a cloth.
36
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Table of contents
- 5 Abbreviations Used in The Manual
- 6 How to use this instruction manual
- 7 Contents
- 11 ON/OFF
- 11 Turning on the microscope
- 11 Supply
- 11 Turning on the fluorescence attachment light source (mercury lamp)
- 12 Adjustment
- 12 Adjustment using the brightness control knob
- 13 Adjustment using the preset switch
- 13 Adjustment with the ND filter IN/OUT lever
- 13 Adjustment with the ND filter for the fluorescence attachment
- 14 Transmitted image in fluorescence observation
- 14 Adjusting the Epi-fluorescence Aperture Diaphragm
- 14 Camera adjustment (adjusting the brightness of the image on the monitor)
- 15 Optical Path Switching
- 15 distribution
- 16 Disabling the clicking of the optical path switching lever
- 17 Motion
- 17 actions
- 17 Knob rotation direction and stage motion direction
- 17 Number of knob turns and distance of stage travel
- 18 Adjusting the rotating torque of the coarse focus knob
- 18 How to Use the Coarse Focus Upper-limit Clamp
- 19 Lateral Stage Motion
- 19 action
- 19 Knob rotation direction and stage motion direction
- 19 Adjusting the knob heights
- 19 Adjusting the knob rotation torque
- 20 rotation
- 20 Stage with Rotating Mechanism
- 20 Centering the Stage
- 20 Magnification
- 21 Adjustment
- 22 Adjusting the Observation Position
- 23 Adjusting the Condenser Position
- 24 Adjusting the Aperture Diaphragm
- 24 12.1 Adjusting the aperture diaphragm opening using the condenser scale
- 25 Selecting a Condenser
- 25 Adjusting the Field Diaphragm
- 26 Operation
- 27 Immersion
- 28 Observation
- 28 17.1 Warning
- 28 17.2 Epi-illumination attachment shutter
- 28 17.3 Light shielding plate
- 29 17.4 Field diaphragm of the Epi-illumination attachment
- 30 Diaphragm
- 31 17.6 Switching Excitation Methods
- 32 17.7 Epifluorescent ND Filters
- 33 Filters
- 33 18.1 Selecting excitation filters (EX filters)
- 34 18.2 Selection of barrier filter (BA filter)
- 35 18.3 Replacing excitation and barrier filters
- 36 Excitation Light Balancer
- 38 Capture
- 38 20.1 Adjusting light intensity
- 38 20.2 Adjusting the condenser
- 38 20.3 Confirming the photomicrographic range
- 38 focus
- 38 20.5 Making adjustments to keep out extraneous light
- 39 photomicrography