Eclox Manual

Table of Contents

Section 1 Specifications......................................................................................... 7

Section 2 General information............................................................................... 9

2.1 Safety information ............................................................................................... 9

2.1.1 Use of hazard information......................................................................... 9

2.2 Product overview............................................................................................... 10

2.2.1 Test descriptions ..................................................................................... 10

2.2.2 Test setup ............................................................................................... 12

2.3 Unpack the instrument ...................................................................................... 12

Section 3 Luminometer ........................................................................................ 13

3.1 Overview ........................................................................................................... 13

3.2 Prepare the luminometer for use....................................................................... 14

3.2.1 Test the operation ................................................................................... 14

3.2.2 Erase the results saved on the luminometer........................................... 15

3.2.3 Set the measurement range ................................................................... 16

3.3 Change the default settings .............................................................................. 18

3.3.1 Set the LCD contrast .............................................................................. 18

3.3.2 Set the waiting time and measuring time ................................................ 18

3.4 Connect the luminometer to a printer................................................................ 19

3.5 Connect the luminometer to a computer ........................................................... 21

3.6 Install LUMISsoft on the computer .................................................................... 21

Section 4 Chemiluminescence Toxicity Test...................................................... 23

4.1 Overview ........................................................................................................... 23

4.2 Prepare the reagents for luminometer calibration and sample testing.............. 23

4.2.1 Prepare CT Reagent 2............................................................................ 24

4.2.2 Prepare CT Reagent 3............................................................................ 24

4.3 Calibrate the luminometer ................................................................................. 26

4.4 Measure pollutants in the water sample............................................................ 28

4.5 Show the previous results ................................................................................. 31

4.6 Send previous results to a computer................................................................. 31

Section 5 EZ Arsenic Test Kit .............................................................................. 33

5.1 EZ Arsenic, 0–500 ppb (0, 10, 25, 50, 100, 250, 500) ...................................... 33

5.2 EZ Arsenic, 0–4000 ppb (0, 35, 75, 175, 1500, 4000) ..................................... 34

5.3 Interferences ..................................................................................................... 34

5.3.1 Removing sulfide (optional) .................................................................... 35

Section 6 Pesticide/Nerve Agent Test ................................................................. 37

6.1 Pesticide/Nerve Agent procedure ..................................................................... 37

EcloxTOC.fm

Table of Contents

Page 3

Table of Contents

Section 7 Free and Total Chlorine Test................................................................39

7.1 Measuring hints and general information...........................................................39

7.2 Free chlorine procedure, 0–3.5 mg/L.................................................................39

7.3 Total chlorine procedure, 0–3.5 mg/L ................................................................41

Section 8 Color Test ..............................................................................................43

8.1 Color Test, low range .........................................................................................43

8.2 Color Test, high range........................................................................................44

Section 9 Pocket Pal™ pH Tester.........................................................................45

9.1 Use and care of the tester .................................................................................45

9.2 2-point calibration ..............................................................................................46

Section 10 Pocket Pal™ TDS Tester ....................................................................47

10.1 Use and care of the tester ...............................................................................47

10.2 Calibration........................................................................................................48

Section 11 Luminescent Bacteria Toxicity Test:

Screening and LIMIT measure..............................................................................49

11.1 Overview ..........................................................................................................49

11.2 Accuracy ..........................................................................................................50

11.3 Reagent description .........................................................................................50

11.3.1 Quality assurance test ...........................................................................50

11.4 Reagent storage and preservation...................................................................51

11.5 Prepare the reagent .........................................................................................51

11.6 Prepare the stock suspension using the LCK491 reagent...............................51

11.7 Prepare the test suspension ............................................................................54

11.8 Sample collection, storage and preservation ...................................................55

11.9 Interferences ....................................................................................................55

11.10 Prepare the sample........................................................................................56

11.11 Prepare the test tubes....................................................................................57

11.12 Measure the toxicity of the sample dilutions ..................................................60

11.13 Show or send previous results.......................................................................65

11.13.1 Description of screening luminescence results....................................65

11.13.2 Description of LIMIT measure results ..................................................66

11.13.3 Show or send screening luminescence results....................................66

11.13.4 Show or send LIMIT measure results ..................................................67

Section 12 Luminescent Bacteria Toxicity Test

according to ISO 11348 part 3 ..............................................................................69

12.1 Overview..........................................................................................................70

12.2 Accuracy ..........................................................................................................71

Table of Contents

Page 4

EcloxTOC.fm

Table of Contents

12.3 Thermostat and PC software requirements .................................................... 72

12.4 Reagent description ........................................................................................ 73

12.5 Reagent storage and preservation.................................................................. 74

12.6 Prepare the reagent ........................................................................................ 75

12.6.1 Prepare the stock suspension using the LCK491 reagent.................... 75

12.6.2 Prepare the test suspension ................................................................. 76

12.7 Sample collection, storage and preservation .................................................. 82

12.8 Interferences ................................................................................................... 83

12.9 Prepare the sample......................................................................................... 84

12.10 Prepare the dilutions series........................................................................... 85

12.10.1 Prepare a 9 dilution series (D 2 values and higher)............................ 85

12.10.2 Prepare a 3 dilution series (D 2 values and higher)............................ 89

12.10.3 Prepare a 9 dilution series (D 1 values and higher)............................ 93

12.10.4 Prepare a 3 dilutions series (D 1 values and higher) .......................... 98

12.11 Measure the light intensity of the test suspension....................................... 101

12.12 Measure the light intensity of the test suspension after the sample dilutions are added ...................................................................... 106

12.13 Show or send previous results .................................................................... 108

Section 13 Maintenance ......................................................................................111

13.1 General maintenance.....................................................................................111

13.1.1 Cleaning the kit ....................................................................................111

13.1.2 Cleaning the luminometer....................................................................111

13.2 Decontamination ............................................................................................111

13.3 Battery replacement...................................................................................... 112

13.3.1 Luminometer battery replacement ...................................................... 112

13.3.2 Pocket Pal™ battery replacement (pH and TDS) ............................... 112

Section 14 Troubleshooting............................................................................... 115

Section 15 Replacement parts and accessories .............................................. 117

EcloxTOC.fm

Table of Contents

Page 5

Table of Contents

Table of Contents

Page 6

EcloxTOC.fm