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90
GB Methods
POLY
0.0.0
Polyacrylate with Liquid reagent
1 – 30 mg/l Polyacrylate
Fill a clean vial (24 mm Ø) with 10 ml of the water sample and perform zero calibration (see “Operation”).
Fill the vial with drops of the same size by holding the bottle vertically and squeeze slowly:
1 ml (25 drops) KS255 (Polyacrylate reagent 1) (note 1).
!
Zero
Test
POLY
RESULT
Close the vial tightly with the cap and invert several times to mix the contents.
Fill the vial with drops of the same size by holding the bottle vertically and squeeze slowly:
1 ml (25 drops) KS256 (Polyacrylate reagent 2)
Close the vial tightly with the cap and invert several times to mix the contents.
Place the vial in the sample chamber making sure that the marks are aligned.
Wait for a reaction period of 10 minutes.
(Countdown can be activated, see page 57)
The method symbol flashes for approx. 3 seconds.
The result is shown in the display in mg/l Polyacrylic Acid 2'100 sodium salt.
MD100_7 12/2016
GB Methods
Notes:
1. If little or no turbidity is present at correct dose concentrations, the sample will need a pre-concentration step in order to detect this level of polyacrylate/polymer. Carry out this procedure as directed then test the pre-concentrated sample as above (see next page).
2. Anomalous results occur when interferences are present as part of the product blend or from sample contaminants. In these instances follow the interference removal steps detailed below and test this treated sample as above (see next page).
3. This test has been calibrated using polyacrylic acid 2'100 sodium salt in the range
1-30 mg/l. Other polyacrylates/polymers will give differing responses and therefore the test range will vary.
Reagent
KS255 (Polyacrylate reagent 1)
KS256 (Polyacrylate reagent 2)
Form of reagent/Quantity Order-No.
Liquid reagent / 65 ml
Liquid reagent / 65 ml
56L025565
56L025665
MD100_7 12/2016 91
GB Methods
Interference removal and Pre-Concentration
Cartridge Preparation
1. Remove the plunger of the 20 ml syringe from the barrel and attach the C18 cartridge.
2. Add 5 ml of KS336 (Propan-2-ol) to the syringe barrel, attach the plunger and pass dropwise through the cartridge. Discard the eluent to waste.
3. Remove plunger and fill the syringe barrel with 20 ml of deionised/tap water. Attach the plunger and pass dropwise through the cartridge. Discard the eluent to waste. The cartridge is now ready to be used/reused.
Interference removal
1. Transfer exactly 20 ml of sample water to a 100 ml sample bottle and dilute to approximately 50-60 ml with deionised water or tap water.
2. Add drops of KS173 (2,4 Dinitrophenol) until a pale yellow colour is observed in the sample.
3. Add drops of KS183 (Nitric Acid) until the yellow colour JUST disappears.
4. Remove the plunger from the barrel of the 60ml plastic syringe and firmly attach the prepared C18 cartridge (see: Cartridge Preparation) to the end of the barrel.
5. Transfer the 50-60 ml of sample from the bottle to the syringe barrel and attach the plunger. Depress the plunger and allow the sample to flow dropwise from the cartridge.
Do not use excessive force to elute the sample quickly. LEAVE THE C18 CARTRIDGE
ATTACHED and remove the plunger. Discard all of eluted sample to waste.
6. Using the 20 ml syringe, add exactly 20 ml of deionised/tap water to the 60 ml syringe barrel attached to the cartridge followed by 1 ml (25 drops) of KS255 (Polyacrylate Reagent 1). Gently swirl the syringe to mix.
7. Attach the plunger and depress. Collect the eluted sample in a clean vessel. Allow the sample to flow dropwise from the cartridge. Do not use excessive force to elute the sample quickly.
8. Add 10 ml of the eluted water sample into clean vial (24 mm Ø).
9. Using this vial perform the measurement of the method polyacrylate (see page 90).
92 MD100_7 12/2016
GB Methods
Pre-Concentration
Pre-concentration uses exactly the same procedure as interference removal, except a greater volume of sample is used in step 1, instead of deionised/tap water.
For calculation of the original sample concentration a concentration factor should be considered:
If a 50 ml sample is used the concentration factor is 20/50 = 0.4
If a 100 ml sample is used the concentration factor is 20/100 = 0.2
This can be extended as required in order to concentrate the polyacrylate/polymer sufficiently for analysis.
Example:
If the reading is 20 mg/l and 50 ml are taken for pre-concentration the original concentration should be calculated as 20 * 0.4 = 8 mg/l.
Note:
Samples exceeding 10,000 TDS should be diluted prior to loading onto the cartridge. Take this dilution into consideration when working out the overall concentration factor.
Reagent / Accessories
KS255 (Polyacrylate reagent 1)
KS256 (Polyacrylate reagent 2)
KS336 (Propan-2-ol)
C18-cartridge
KS173 (2,4 Dinitrophenol)
KS183 (Nitric Acid)
Form of reagent/Quantity Order-No.
Liquid reagent / 65 ml
Liquid reagent / 65 ml
Liquid reagent / 65 ml
Liquid reagent / 65 ml
Liquid reagent / 65 ml
56L025565
56L025665
56L033665
AS-K22811-KW
56L017365
56L018365
MD100_7 12/2016 93
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