- No category
advertisement
Bravo Automated Liquid-Handling Platform
User Guide
C
Setting up the Barcode Reader
This section contains the following topics:
•
“About setting up the Barcode Reader” on page 166
•
“Installing or removing the Barcode Reader” on page 168
•
“Creating a profile for the Barcode Reader” on page 169
•
“Specifying the Barcode Reader location” on page 171
•
“Testing and optimizing barcode scanning” on page 173
165
C Setting up the Barcode Reader
About setting up the Barcode Reader
About setting up the Barcode Reader
About this topic
This topic describes the Barcode Reader and provides the workflow for setting up the Barcode Reader. To operate the Barcode Reader, see the
VWorks
Automation Control User Guide
.
Barcode Reader description
The Barcode Reader consists of a platepad with a barcode reader sensor head
(under the platepad) and a mirror. The Barcode Reader can be installed in place of a platepad at any deck location and is designed to scan barcodes on the east or west side of microplates.
Figure Barcode Reader oriented to scan barcodes on the east side of microplates
Mirror
00274
Overall Bar Code Reader with mirror
166
Platepad Barcode reader sensor head
(under the platepad)
Setup workflow
The following table presents the workflow for installing and setting up the
Barcode Reader. The procedures can vary depending on the brand of barcode reader sensor head (for example, Keyence or Microscan).
Step Procedure
1
2
3
4
Verify the barcode requirements.
Install the Barcode Reader.
See...
“Barcode label specifications” on page 167
“Installing or removing the Barcode
Add the Barcode Reader device and create a profile for the Barcode Reader.
“Creating a profile for the Barcode
Specify the Barcode Reader location in the Bravo device file.
“Specifying the Barcode Reader location” on page 171
Bravo Automated Liquid-Handling Platform User Guide
C Setting up the Barcode Reader
About setting up the Barcode Reader
Step Procedure
5
See...
Adjust the Barcode Reader for optimal scanning.
“Testing and optimizing barcode scanning” on page 173
Barcode label specifications
The barcode reader can read barcodes that meet the following requirements:
Characteristic
Barcode height
Print contrast
Quiet zone
Keyence requirements
Barcode symbology Code 39
Code 128
Interleaved 2 of 5
Code 93
Microscan requirements
Code 39
Codabar
Code 128
Interleaved 2 of 5
Code 93
Code 2 of 5
3.34 mm (0.13 in) or taller
25% at 650 nm
3.34 mm (0.13 in) or taller
25% at 650 nm
10 times the dimension of the narrowest element in the barcode or 0.25 in, whichever is greater
10 times the dimension of the narrowest element in the barcode or 0.25 in, whichever is greater
Note: The symbology must be stored in the barcode reader in order for the barcode reader to decode the barcode symbol.
Related information
For information about...
Setting up other accessories
Using an accessory in a protocol
See...
•
“Accessories and platepads” on page 101
•
“Autofilling accessories” on page 155
VWorks Automation Control User
Guide
Bravo Automated Liquid-Handling Platform User Guide 167
C Setting up the Barcode Reader
Installing or removing the Barcode Reader
Installing or removing the Barcode Reader
Before you start
Make sure you have the following:
• Barcode Reader
• M4 hex wrench
• Accessories Hub
Procedures
WARNING
Turn off the Bravo Platform before you install or remove the accessory.
To install the Barcode Reader:
1
Using the M4 hex wrench, remove the screw in the center of the platepad and remove the platepad.
2
Place the Barcode Reader at the same deck location in the correct orientation to scan barcodes on the west side or the east side of microplates. Make sure it sits level on the deck.
3
Insert the supplied flathead cap screw into the center of the Barcode
Reader platepad and use the M4 hex wrench to tighten the screw.
4
Connect the cable from the Barcode Reader to the Barcode Reader module port on the Accessories Hub.
5
Edit the teachpoint for this location.
To remove the Barcode Reader:
1
Disconnect the Barcode Reader cable from the Accessories Hub.
2
Using the M4 hex wrench, remove the screw in the center of the Barcode
Reader and remove the Barcode Reader.
3
Place a platepad at the same deck location.
4
Insert the supplied flathead cap screw into the center of the platepad and use the M4 hex wrench to tighten the screw.
Related information
For information about...
Barcode specifications
Creating a profile for the Barcode
Reader
Specifying the Barcode Reader location in the device file
See...
“About setting up the Barcode Reader” on page 166
“Creating a profile for the Barcode
“Specifying the Barcode Reader location” on page 171
Making adjustments and troubleshooting the Barcode Reader
“Testing and optimizing barcode scanning” on page 173
168 Bravo Automated Liquid-Handling Platform User Guide
C Setting up the Barcode Reader
Creating a profile for the Barcode Reader
Creating a profile for the Barcode Reader
About this topic
This topic explains how to add the Barcode Reader device, and then create and initialize a profile for the Barcode Reader.
For details on creating a device file or adding devices to a device file, see
“Creating or adding a Bravo device” on page 21
.
Procedure
To add the Barcode Reader device and create a profile:
1
In the VWorks window, create a new or open an existing Bravo device file.
2
In the Available Devices area, double- click the icon for your barcode reader sensor head (Keyence or Microscan). The Barcode Reader icon appears in the Devices area.
3
In the
Devices area, select the Barcode Reader icon, and click Device
diagnostics. The Barcode Reader Diagnostics dialog box opens.
Bravo Automated Liquid-Handling Platform User Guide 169
C Setting up the Barcode Reader
Creating a profile for the Barcode Reader
Figure Keyence Barcode Reader Diagnostics dialog box
Figure Microscan Barcode Reader Diagnostics dialog box
170
4
In the Profiles tab under Profile Management, click Create a new profile. The
Create Profile dialog box opens.
5
Type a name for the new profile and click
OK. The new profile name appears in the Profile name list.
6
In the Profile Settings area, select the Accessories Hub Serial port that is connected to the Barcode Reader.
7
Microscan only. In the
Default barcode side list, select East or West to indicate the side of the labware that has the barcode label.
Note: The Bravo Barcode Reader can scan barcode labels on the labware east and west sides only.
Bravo Automated Liquid-Handling Platform User Guide
C Setting up the Barcode Reader
Specifying the Barcode Reader location
8
Click
Update this profile to save the newly created profile.
9
Click
Initialize this profile to establish communication with the Barcode
Reader.
10
In the VWorks software window, choose File > Save and save the device file.
Related information
For information about...
Barcode specifications
Installing the Barcode Reader
Specifying the Barcode Reader location on the Bravo Platform
Making adjustments and troubleshooting the Barcode Reader
Setting up other accessories
See...
“About setting up the Barcode Reader” on page 166
“Installing or removing the Barcode
“Specifying the Barcode Reader location” on page 171
“Testing and optimizing barcode scanning” on page 173
•
“Accessories and platepads” on page 101
•
“Autofilling accessories” on page 155
Specifying the Barcode Reader location
About this topic
Use the following procedure to specify the location of the Barcode Reader in the Bravo device file.
Procedure
To specify the Barcode Reader location in the Bravo device file:
1
Open the Bravo device file that contains the Barcode Reader device.
2
In the Devices area, expand the Bravo device. The list of deck locations appear.
Bravo Automated Liquid-Handling Platform User Guide 171
C Setting up the Barcode Reader
Specifying the Barcode Reader location
3
Select the deck location at which you installed the Barcode Reader. In the example in
the Barcode Reader is installed at deck location 7, so deck location 7 is selected.
4
In the location properties table, select the Barcode Reader for one of the following:
• BCR on west side
• BCR on east side
Note: The Bravo Barcode Reader can scan barcode labels on the labware east and west sides only.
In the following example, the Barcode Reader is at deck location 7, and it will scan barcodes on the west side of microplates.
172
5
When you are finished, click Initialize selected devices to re- establish communication with the Bravo and use the updated device file.
Bravo Automated Liquid-Handling Platform User Guide
C Setting up the Barcode Reader
Testing and optimizing barcode scanning
Related information
For information about...
Barcode specifications
Installing the Barcode Reader
Creating a profile for the Barcode
Reader
Making adjustments and troubleshooting the Barcode Reader
Setting up other accessories
See...
“About setting up the Barcode Reader” on page 166
“Installing or removing the Barcode
“Creating a profile for the Barcode
“Testing and optimizing barcode scanning” on page 173
•
“Accessories and platepads” on page 101
•
“Autofilling accessories” on page 155
Testing and optimizing barcode scanning
About this topic
This topics describes:
•
“Using diagnostics to test the barcode scanning (Keyence sensor)” on page 173
•
“Using diagnostics to test the barcode scanning (Microscan sensor)” on page 175
•
“Adjusting the scan angle” on page 177
•
Before you start
Make sure you have the following:
• M2 hex wrench
• Spare microplate that has a barcode label
Using diagnostics to test the barcode scanning (Keyence sensor)
If the barcode reader has a Keyence sensor head, use the procedure in this section to test the barcode scanning.
WARNING
Class II laser hazard. Looking directly at the laser beam can result in serious eye injury. Do not look directly at the laser beam.
Bravo Automated Liquid-Handling Platform User Guide 173
C Setting up the Barcode Reader
Testing and optimizing barcode scanning
WARNING
Laser emission does not automatically turn off if the sensor head is disassembled. Do not disassemble the barcode reader sensor head.
To test the barcode scanning:
1
Place the spare microplate on the Barcode Reader platepad and make sure the barcode label faces the Barcode Reader mirror.
2
Open
Barcode Reader Diagnostics.
3
In the
Profiles tab, select the Barcode Reader profile from the Profile list, and click
Initialize this profile.
Figure Keyence Barcode Reader Diagnostics Profiles tab
4
Click the Controls tab.
Figure Keyence Barcode Reader Diagnostics Controls tab
174 Bravo Automated Liquid-Handling Platform User Guide
C Setting up the Barcode Reader
Testing and optimizing barcode scanning
5
Click
Scan barcode. The barcode reader laser turns on briefly to scan the barcode label. One of the following messages appears next to Scan result:
Scan result message
<barcode text>
No barcode found
Description
The barcode was successfully scanned.
Barcode scanning was not successful.
Next step
The setup is complete. No further adjustment is required.
6
If the No barcode found message appears, click
Scan barcode again and check that the barcode reader laser beam is projected onto the vertical center of the barcode label.
If the laser beam is not at the vertical center of the label, adjust the scan angle of the Barcode Reader mirror.
Using diagnostics to test the barcode scanning (Microscan sensor)
If the barcode reader has a Microscan sensor head, use the procedure in this section to test the barcode scanning.
WARNING
Class II laser hazard. Looking directly at the laser beam can result in serious eye injury. Do not look directly at the laser beam.
WARNING
Laser emission from the reader does not automatically turn off if the sensor head is disassembled. Do not disassemble the barcode reader sensor head.
To test the barcode scanning:
1
Place the spare microplate on the Barcode Reader platepad and make sure the barcode label faces the Barcode Reader mirror.
2
Open
Barcode Reader Diagnostics.
3
In the Profiles tab, select the Barcode Reader profile from the Profile list, and click Initialize this profile.
Figure Microscan Barcode Reader Diagnostics Profiles tab
Bravo Automated Liquid-Handling Platform User Guide 175
C Setting up the Barcode Reader
Testing and optimizing barcode scanning
4
Click the
Controls tab.
Figure Microscan Barcode Reader Diagnostics Controls tab
176
5
Click Scan. The barcode reader laser turns on briefly to scan the barcode label. One of the following messages appears next to Result:
Scan result message
<barcode text>
No barcode found
Description
The barcode was successfully scanned.
Barcode scanning was not successful.
Next step
The setup is complete. No further adjustment is required.
6
If the No barcode found message appears, click Scan again, and check that the barcode reader laser beam is projected onto the vertical center of the barcode label.
If the laser beam is not at the vertical center of the label, adjust the scan angle of the Barcode Reader mirror.
7
To optimize the scanner settings, click Calibrate.
Note: During the calibration process, the scanner attempts various settings to determine the optimum decode rate for the given conditions.
Bravo Automated Liquid-Handling Platform User Guide
C Setting up the Barcode Reader
Testing and optimizing barcode scanning
Adjusting the scan angle
To adjust the scan angle of the Barcode Reader:
1
Loosen the screws on both sides of the Barcode Reader mirror.
00275
Bar code reader with mirror detail
Loosen the screws on both sides of the mirror.
2
Tilt the Barcode Reader mirror slightly. If the angle is set up properly, you can see the laser beam at the vertical center of the barcode label each time you click Scan barcode.
Tilt
00275
Bar code reader with mirror detail
3
Tighten the screws on both sides of the barcode reader sensor head.
4
Repeat the procedures to test the barcode scanning and adjust the scan angle until the reader is able to scan the barcode successfully (the barcode text appears next to Scan result).
Troubleshooting
If the reader is unable to scan the barcode after many adjustments, try one or more of the following:
• Make sure the barcode label is applied properly onto the microplate.
• Make sure the microplate is sitting level on the Barcode Reader platepad.
Bravo Automated Liquid-Handling Platform User Guide 177
C Setting up the Barcode Reader
Testing and optimizing barcode scanning
• Make sure the barcode meets the requirements. See
“Barcode label specifications” on page 167 .
• Repeat the adjustment process using a new spare microplate.
Related information
For information about...
Barcode specifications
Installing the Barcode Reader
Creating a profile for the Barcode
Reader
Specifying the Barcode Reader location in the device file
Setting up other accessories
See...
“About setting up the Barcode Reader” on page 166
“Installing or removing the Barcode
“Creating a profile for the Barcode
“Specifying the Barcode Reader location” on page 171
•
“Accessories and platepads” on page 101
•
“Autofilling accessories” on page 155
178 Bravo Automated Liquid-Handling Platform User Guide
advertisement
Related manuals
advertisement
Table of contents
- 7 Preface
- 8 About this guide
- 9 Accessing Automation Solutions user information
- 15 Introduction to the Bravo Platform
- 16 Safety notes
- 17 About the Bravo Platform
- 19 Hardware overview
- 23 Connection panel description
- 26 Pipette heads and pin tools
- 29 Software overview
- 31 Setting up the Bravo Platform
- 32 Workflow for setting up the Bravo Platform
- 33 Starting up and shutting down
- 35 Creating or adding a Bravo device
- 40 Opening Diagnostics
- 42 Creating and managing profiles
- 46 Initializing the Bravo Platform
- 50 Setting teachpoints
- 57 Verifying the gripper setup
- 59 Configuring external robot access
- 69 Preparing for a protocol run
- 70 Workflow for preparing a protocol run
- 70 Emergency stops
- 71 Planning for the protocol run
- 72 About performing dry runs
- 73 Maintaining the Bravo Platform
- 74 Routine maintenance
- 75 Cleaning the Bravo Platform
- 76 Cleaning up after a run
- 77 Changing the Bravo head
- 83 Replacing the fuse
- 84 Retracting tip box stripper pins
- 86 Moving the head manually
- 87 Recovering from a head collision
- 89 Troubleshooting hardware problems
- 91 Troubleshooting hardware-related error messages
- 92 Reporting problems
- 95 Using Bravo Diagnostics
- 96 About the Jog/Teach tab
- 98 Homing the pipette head
- 100 Jogging the pipette head
- 102 Changing the pipette head speed
- 103 Using the Move and Approach commands
- 105 Fine-tuning the gripper movements
- 111 Clearing motor faults and checking the head type
- 112 Performing a task using Bravo Diagnostics
- 115 Accessories and platepads
- 116 Accessories overview for the Bravo Platform
- 118 Installing the Accessories Hub
- 121 Setting up a Microplate Vacuum Alignment Station
- 126 Setting up the Orbital Shaking Station
- 132 Using the teach plate to set the teachpoint for an accessory
- 134 Setting up the Vacuum Filtration Station
- 147 Configuring a platepad for delidding
- 148 Installing an Alignment Station
- 150 Setting up a Nested Rack Insert
- 153 Using the Manual Fill Reservoir
- 154 Setting up the Thermal Station
- 157 Setting up thermal and shaking stations (Inheco controller)
- 165 (Bravo SRT only) Setting up an LT tip box location
- 169 Autofilling accessories
- 170 Autofilling station overview and setup workflow
- 171 Configuring an autofilling station for the Bravo Platform
- 176 Setting up a Weigh Station for the Bravo Platform
- 179 Setting up the Barcode Reader
- 180 About setting up the Barcode Reader
- 182 Installing or removing the Barcode Reader
- 183 Creating a profile for the Barcode Reader
- 185 Specifying the Barcode Reader location
- 187 Testing and optimizing barcode scanning
- 193 Setting up the AssayMAP Bravo Platform
- 194 AssayMAP Bravo Platform overview
- 197 Bravo 96AM Head overview
- 202 AssayMAP cartridge overview
- 204 About installing the AssayMAP Bravo Platform
- 205 Installing and using the Bravo 96AM Head
- 213 Installing the 96AM Wash Station
- 217 Setting up the AssayMAP Bravo Platform
- 220 Setting teachpoints for the AssayMAP Bravo Platform
- 223 Verifying and adjusting teachpoints for the accessory locations
- 231 Opening the Protein Sample Prep Workbench
- 232 Requirements for partial-head liquid handling
- 237 Diagnostics quick reference
- 238 Bravo Diagnostics dialog box
- 239 Configuration tab quick reference
- 242 Accessory Diagnostics dialog box
- 243 External Robots tab quick reference
- 244 IO tab quick reference
- 246 Jog/Teach tab quick reference
- 250 Gripper tab quick reference
- 252 Profiles tab quick reference
- 256 Processes tab quick reference
- 259 Aspirate task parameters
- 263 Dispense task parameters
- 265 Dispense to Waste task parameters
- 269 Mix task parameters
- 272 Pump Reagent parameters
- 273 Shake task parameters
- 275 Vacuum Filtration Station task parameters
- 278 Wash Tips task parameters
- 281 Pin Tool task parameters
- 285 AM Aspirate task parameters
- 290 AM Dispense task parameters
- 295 AM Mix task parameters
- 299 Index