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D Setting up the AssayMAP Bravo Platform
AssayMAP Bravo Platform overview
AssayMAP Bravo Platform overview
This topic provides an overview of the platform components and standard hardware configurations.
Hardware components overview
The AssayMAP Bravo Platform can enable high- throughput microchromatography for small- scale sample preparation. The following figure shows the components required for the AssayMAP Bravo Platform. The Bravo
Platform must include the gripper assembly, which is required for removing the mounted AssayMAP cartridges and disposable tips from the Bravo 96AM Head.
For a detailed description of the Bravo Platform and gripper assembly, see
.
Figure AssayMAP Bravo Platform required components
180
Item Name
1
Bravo 96AM Head
Description
The 96- channel head that can transfer liquids with the bare probes of the syringes, through AssayMAP cartridges mounted on the probes, and using 250- µL disposable tips mounted on the probes. For a
detailed description, see Bravo 96AM Head overview .
Bravo Automated Liquid-Handling Platform User Guide
D Setting up the AssayMAP Bravo Platform
AssayMAP Bravo Platform overview
Item Name
2
Description
AssayMAP cartridge The microchromatography cartridge for small- scale sample preparation. Each cartridge features a 5- µL bed that is packed with chromatographic resin to support a variety of applications. Liquid can be aspirated or dispensed through the packed resin in the cartridges.
The AssayMAP Bravo Platform provides the precision flow- rate control required for aspirating or dispensing the fluid through the packed resin in the cartridges.
For a detailed description, see
3
4
96AM Cartridge &
Tip Seating Station
AssayMAP autofilling station
Pump
Module 2.0
The metal rack that holds the AssayMAP cartridges during runs and supports the cartridges when they are mounted on the probes. The seating station is also required for mounting fewer than 96 tips on the head.
Note: The Cartridge Transfer Utility protocol transfers the cartridges automatically from the conventional cartridge rack into the seating station.
The autofilling station that consists of the Pump Module 2.0, tubing kit, and 96AM Wash Station.
The Pump Module supplies liquid to the 96AM Wash Station for washing operations, and it removes the waste liquid during washing operations.
Tubing kit with inline filter
Ensure that the inline filter is connected in the input tubing line between Pump Module and the 96AM Wash Station to help prevent any tubing particulates from potentially clogging the chimneys of the 96AM Wash Station.
96AM Wash
Station
The 96- chimney reservoir for washing the probes, mounted cartridges, and mounted tips of the Bravo 96AM Head. The chimneys in the wash station prevent carryover and reduce contamination. The liquid enters the 96AM Wash Station through two input ports and then flows up through the chimneys to wash the cartridges, tips, or probes. The waste overflows from the chimneys and is removed through two outlet ports.
CAUTION
Using a model of the Tip Wash Station other than the 96AM Wash Station can result in a potential crash with the Bravo 96AM Head. Ensure that you use only the 96AM
Wash Station.
Configuration options to support AssayMAP applications
Different AssayMAP applications require slightly different hardware configurations. The following figure and table describe the two standard hardware configurations.
Bravo Automated Liquid-Handling Platform User Guide 181
D Setting up the AssayMAP Bravo Platform
AssayMAP Bravo Platform overview
Figure Standard configurations: (
A
) basic and (
B
) enhanced
182
Configuration AssayMAP Bravo Platform with…
A
Basic
B
Enhanced
AssayMAP applications
96AM Wash Station (
1
) (autofilling station) at deck location 1
Standard applications that do not require temperature control, such as Antibody
Purification
• 96AM Wash Station (autofilling station) (
1
) at deck location 1
• Peltier Thermal Station (
2
) with a custom plate nest and PCR plate insert at deck location 4
Bravo risers are required to accommodate the height
• Orbital Shaking Station (
3
) at deck location 9
Applications that require temperature control, such as
N- glycan Sample Prep or In- Solution Digestion
Software overview
The Protein Sample Prep Workbench is an interface that provides a collection of AssayMAP workflows, protocols, and forms for running protein sample prep applications on the AssayMAP Bravo Platform. The Protein Sample Prep
Workbench includes the following:
• Workflow Library
• Application Library
• Utility Library
• Literature Library
The VWorks software is required for the Protein Sample Prep Workbench. You set up the AssayMAP Bravo Platform using the VWorks software.
Bravo Automated Liquid-Handling Platform User Guide
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