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D Setting up the AssayMAP Bravo Platform
Verifying and adjusting teachpoints for the accessory locations
About teaching accessory locations on the AssayMAP Bravo Platform
The AssayMAP Bravo Platform can include the following accessories, which have additional teaching requirements.
• Deck location 1, 96AM Wash Station (all configurations)
• Deck locations 2 and 4, Bravo Plate Riser (Normalization application)
• Deck location 4, Peltier Thermal Station (enhanced configuration)
• Deck location 9, Orbital Shaking Station (enhanced configuration)
To set the teachpoints for the accessory locations, see
“Verifying and adjusting teachpoints for the accessory locations” on page 209
.
Related information
For more information about… See…
Entering labware definitions in the
VWorks software
VWorks Automation Control Setup
Guide
Setting teachpoints for accessories on the AssayMAP Bravo Platform
“Verifying and adjusting teachpoints for the accessory locations” on page 209
Verifying and adjusting teachpoints for the accessory locations
About this topic
This topic describes how to verify and adjust the teachpoints for the accessory locations on the AssayMAP Bravo Platform, including:
•
“Setting and verifying the teachpoint for the Bravo Plate Riser” on page 210
•
“Teaching the Peltier Thermal Station at deck location 4” on page 212
•
“Teaching the Orbital Shaking Station at deck location 9” on page 213
•
“Verifying and adjusting the teachpoint for the 96AM Wash Station” on page 214
Before you start
WARNING
To avoid potential injury and equipment damage, only personnel trained in how to teach the Bravo Platform should perform the procedure in this topic.
WARNING
If you touch any of the moving parts or attempt to move labware while the
Bravo Platform is in operation, the device could pinch, pierce, or bruise you. Keep your fingers, hair, clothing, and jewelry away from the device while it is in motion.
WARNING
When you initialize the Bravo Platform, the head and tie bar can move. To prevent injury, keep clear of the device while it is in motion.
Bravo Automated Liquid-Handling Platform User Guide 209
D Setting up the AssayMAP Bravo Platform
Verifying and adjusting teachpoints for the accessory locations
CAUTION
To avoid potential hardware crashes that can damage your equipment, make sure that you verify the accuracy of any teachpoints before the system is used to run protocols.
CAUTION
To prevent the head from crashing into an accessory, be sure to start the accessory teaching process from the safe z-axis point above the accessory location. This is especially important if you use the Set all teachpoints based upon selected teachpoint feature.
Ensure the following:
• The 96AM Wash Station is installed at deck location 1. The deck is clear of any labware.
• The initial teachpoints have already been derived using the procedure in
“Setting teachpoints for the AssayMAP Bravo Platform” on page 206
.
Setting and verifying the teachpoint for the Bravo Plate Riser
The Bravo Plate Riser is a 2.84- cm tall deck accessory that you can place atop a standard platepad on the Bravo deck. The Bravo Plate Riser requires a teachpoint adjustment in the Bravo profile to ensure that the Bravo head moves to the correct height at the location where the Bravo Plate Riser is installed.
The following figure shows the Bravo Plate Riser, which has a crosshairs in one corner to facilitate adjusting the teachpoint.
Figure Bravo Plate Riser
210
CAUTION
Placing the Bravo Plate Riser at a deck location that does not have the corresponding teachpoint in the device profile will result in a collision, causing equipment damage. Ensure that the device profile includes the corresponding teachpoint for any deck location where the Bravo Plate Riser is installed.
The device file for the AssayMAP Normalization application (Normalization- AM
Bravo.dev) includes the teachpoints for the Bravo Plate Riser at deck locations 2 and 6. However, you should adjust the teachpoints on your instrument and verify the teachpoints before running the application for the first time.
Bravo Automated Liquid-Handling Platform User Guide
D Setting up the AssayMAP Bravo Platform
Verifying and adjusting teachpoints for the accessory locations
Figure Normalization application Bravo Plate Riser deck locations
1
4
00240
Bravo
Plate Orientation
2
5
3
6
7 8 9
00240
IMPORTANT
When setting the teachpoint, ensure that a 250- µL pipette tip is seated on probe A1 (back left corner) of the Bravo 96AM Head.
Adjusting the teachpoint for the Bravo Plate Riser
Use the following procedure to adjust a standard platepad teachpoint to accommodate the Bravo Plate Riser.
To adjust the teachpoint for the Bravo Plate Riser:
1
Place the Bravo Plate Riser on the platepad at the corresponding deck location. Ensure that the Bravo Plate Riser is oriented so that the crosshairs is at the back left corner.
Press down on the Bravo Plate Riser so that it is seated on the platepad.
2
In the
Jog/Teach tab, click the Location list, and then select the deck location that you are teaching.
3
In the Approach height above teachpoint box, type 50, and then click Approach.
The Bravo head moves to the selected deck location, and then moves down so that the tip is approximately 22 mm above the crosshairs on the Bravo
Plate Riser.
4
Carefully, use the
Jog Axes controls to move the head down in smaller and smaller increments until the pipette tip is about 1 mm above the crosshairs on the Bravo Plate Riser.
Visually inspect the alignment to determine if the left- to- right (X) and front- to- back (Y) positions of the tip are centered above the crosshairs on the Bravo Plate Riser. If necessary, use the
Jog Axes controls (Left, Right and Forward, Back) to center the A1 tip above the crosshairs.
5
Carefully, use the
Jog Axes controls to move the head down in tiny increments until the pipette tip is as close as possible to the crosshairs on the Bravo Plate Riser without touching the surface.
6
Click
Teach to set the new teachpoint values for the deck location.
7
To save the new teachpoint for this location, click the
Profiles tab, and then click
Update this profile.
8
Repeat this procedure for any other deck location where the Bravo Plate
Riser will be installed.
Bravo Automated Liquid-Handling Platform User Guide 211
D Setting up the AssayMAP Bravo Platform
Verifying and adjusting teachpoints for the accessory locations
Verifying the teachpoint for the Bravo Plate Riser
Use the following procedure to verify the adjusted teachpoint for the Bravo
Plate Riser.
1
Ensure that the Bravo Plate Riser is securely seated on the platepad at the corresponding deck location. Ensure that the Bravo Plate Riser is oriented so that the crosshairs is at the back left corner.
2
In the Jog/Teach tab, click the Location list, and then select the deck location you are teaching.
3
Click
Move, The Bravo head moves to the selected deck location so that the tip is at the crosshairs on the Bravo Plate Riser.
4
Visually inspect the alignment to ensure that the tip is centered from left to right and front to back.
CAUTION
A hardware collision can occur if you initialize a device profile that does not include the teachpoints for the Bravo Plate Riser while the Bravo Plate Riser remains on the deck. Ensure that you remove the Bravo Plate Riser from the deck when initializing a different device profile.
Teaching the Peltier Thermal Station at deck location 4
The AssayMAP GlykoPrep- plus applications require the red PCR plate insert to be present in the plate nest of the Peltier Thermal Station for the entire workflow. Use the following teaching procedure to accommodate this requirement for deck location 4 on the AssayMAP Bravo Platform.
Figure PCR plate insert
IMPORTANT
Ensure that the PCR plate insert is not installed in the Peltier
Thermal Station at deck location 4 for the following teaching procedure.
IMPORTANT
Ensure that a 250- µL pipette tip is seated on probe A1 (back left corner) of the Bravo 96AM Head.
To adjust the teachpoint for the PCR plate insert:
1
In the Jog/Teach tab, click Location 4.
212 Bravo Automated Liquid-Handling Platform User Guide
D Setting up the AssayMAP Bravo Platform
Verifying and adjusting teachpoints for the accessory locations
2
Click Move to move the head to the previously defined teachpoint for location 4.
3
Using the
Jog Axes controls, jog the head up (- Z) by exactly 4.3 mm.
For example, if the value for the teachpoint’s Z- axis coordinate from the fine tuning was 104.00 mm, the new value after adjusting by 4.3 mm will be 99.70 mm.
4
Click Teach to set the new teachpoint.
5
To save the new teachpoint for this location, click the Profiles tab, and then click
Update this profile.
Teaching the Orbital Shaking Station at deck location 9
The Orbital Shaking Station is required at deck location 9 for the AssayMAP
GlykoPrep- Plus profile configuration. Although the Orbital Shaking Station might not be required for applications other than the GlykoPrep- Plus workflows, if the Orbital Shaking Station is installed at deck location 9, a teachpoint adjustment is required. Use the following procedure to set the teachpoint for the Orbital Shaking Station at deck location 9.
Before you start
Ensure that you have the pipettor teaching plate (G5550- 17692). The teaching plate is a 10- mm thick metal plate that fits in the plate nest of an accessory.
The teaching plate has a crosshairs in one corner, as the following figure shows.
Bravo Automated Liquid-Handling Platform User Guide 213
D Setting up the AssayMAP Bravo Platform
Verifying and adjusting teachpoints for the accessory locations
Figure Pipettor teaching plate
214
IMPORTANT
Ensure that a 250- µL pipette tip is seated on probe A1 (back left corner) of the Bravo 96AM Head.
To teach the Orbital Shaking Station at deck location 9:
1
Place the teaching plate in the plate nest of the Orbital Shaking Station.
Ensure that the crosshairs is at the back left corner and facing upward.
Press down on the teaching plate so that the bottom touches the top surface of the plate nest.
2
In the Jog/Teach tab, and click Location 9.
3
In the Approach height above teachpoint box, type 20, and then click Approach.
The Bravo head moves to deck location 9 and moves down so that the tip is approximately 10 mm above the teaching plate crosshairs.
4
Carefully, use the Jog Axes controls to center the A1 tip above the crosshairs on the teaching plate. When the X and Y positions are properly centered, click
Teach.
5
Click the Move to safe height at current X/Y position. Remove the teaching plate from the Orbital Shaking Station.
6
Ensure that deck location 9 is still selected, and then click
Move. The head moves down so that the tip is approximately 10 mm above the top surface of the plate nest in the Orbital Shaking Station.
7
Carefully, use the
Jog Axes controls to move the head down in the z- axis until the pipette tip is as close as possible to the top surface of the plate nest, without making contact.
8
Click
Teach to set the new teachpoint values for deck location 9.
9
To save the new teachpoint for this location, click the
Profiles tab, and then click
Update this profile.
Verifying and adjusting the teachpoint for the 96AM Wash Station
CAUTION
Failure to remove the pipette tip from the A1 probe will cause the pipette tip to collide with the wash station chimney, damaging the Bravo 96AM Head and 96AM Wash
Station. Ensure that no pipette tips or cartridges are mounted on the probes before starting the procedure.
To verify the 96AM Wash Station teachpoint:
1
In Bravo Diagnostics, click the Jog/Teach tab, and then select Location 1.
The Pump Module icon at location 1 is highlighted and the teachpoint coordinates are displayed in the
X-axis, Y-axis, and Z-axis boxes.
Bravo Automated Liquid-Handling Platform User Guide
D Setting up the AssayMAP Bravo Platform
Verifying and adjusting teachpoints for the accessory locations
2
Write down the
Z-axis value on a piece of paper for future use.
Note: The Z- axis teachpoint value is typically between 105.2—105.8 mm for a standard platepad at deck location 1 on a standard AssayMAP
Bravo Platform.
3
In the Approach height above teachpoint (mm) box, select 50, and then click
Approach. The Bravo head moves into position above the wash station at deck location 1.
4
Carefully, use the Jog Axes controls to position the syringe probes so that they are perfectly centered above the corresponding chimneys in the wash station.
The tips of the probes should be approximately 1 mm above the tops of the chimneys.
5
Perform the following steps to jog the probes down into the chimneys to the original z- axis coordinate that you recorded in
:
CAUTION
Jogging the head down too far will damage the head. Be careful not to jog the head down too far.
Bravo Automated Liquid-Handling Platform User Guide 215
D Setting up the AssayMAP Bravo Platform
Verifying and adjusting teachpoints for the accessory locations
CAUTION
When jogging down in the z-axis, a warning might appear stating that you are about to exceed the set teachpoint, which could cause a collision. Before you click
OK, verify that enough space remains to continue jogging down.
a
In the Jog Axes area, use the Down +Z control to jog in progressively smaller increments as the head gets closer to the deck.
b
Keep track of the changing
Z-Axis readout in the lower right corner of the
Jog/Teach tab.
c
When the Z-Axis readout matches the Z- axis value recorded in
click Teach to store the new coordinates as the teachpoint for deck location 1.
216
6
Click the
Move to safe height at current X/Y position to remove the probes from the wash station chimneys, and return the head to the safe z- axis height.
7
To save the new teachpoint for this location, click the Profiles tab, and then click
Update this profile.
Related information
For more information about… See…
Installing the 96AM Wash Station
“Installing the 96AM Wash Station” on page 199
Bravo Automated Liquid-Handling Platform User Guide
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